Part:BBa_K4998019

LipA-Tat Pathway

Usage and Biology

This part contains the sequence for the LipA signal peptide of the twin-arginine translocation (Tat) Pathway. This pathway is one of the three that have been identified in bacteria and is responsible for the transport of folded proteins extracellularly. [1] The system recognizes an N-terminal signal and translocates these specific proteins. The signal peptide that is chosen affects the level of secretion of the protein that it is connected to, but studies have shown that different proteins have different optimal signal peptides. LipA is an N-terminal signal sequence that is originated by Bacillus subtilis and consists of 31 amino acids. [2] We chose LipA to ensure the secretion of one of our proteins, lac7.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Characterization

The results of this part are shown in BBa_K4998018.

References

[1] Monteferrante, C. G., MacKichan, C., Marchadier, E., Prejean, M.-V., Carballido-López, R., & van Dijl, J. M. (2013). Mapping the twin-arginine protein translocation network of Bacillus subtilis. PROTEOMICS, 13(5), 800–811. https://doi.org/10.1002/pmic.201200416

[2] Zhang, W., Yang, M., Yang, Y., Zhan, J., Zhou, Y., & Zhao, X. (2016). Optimal secretion of alkali-tolerant xylanase in bacillus subtilis by signal peptide screening. Applied Microbiology and Biotechnology, 100(20), 8745–8756. https://doi.org/10.1007/s00253-016-7615-4